Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

Oct 24, 2013 · This protocol provides experimentally derived guidelines for the selection of target sites, evaluation of cleavage efficiency and analysis of off-target activity.

Uncover the secrets of CRISPR Screening with this step-by-step guide. Learn how to select gene editing tools, design gRNA datasets, construct libraries, and more. Dive in now to enhance your CRISPR …

These step-by-step CRISPR protocols have been optimized for maximum efficiency, viability, and reproducibility across a broad range of cell types and gene targets.

A basic CRISPR-Cas9 protocol relies on two main components: the Cas9 enzyme and a guide RNA (gRNA). Cas9 acts as molecular scissors, cutting DNA at a precise location.

To address these challenges, we present an integrated computational protocol with easy-to-do steps for researchers to guide the rational design of CRISPR/Cas variants with improved stability and specificity.

Learn the complete CRISPR gene editing protocol with our detailed guide covering target selection, gRNA design, DNA cleavage, and validation steps for successful genetic modifications

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. The discovery of the type II prokaryotic CRISPR “immune system” has allowed for the development for an RNA-guided …

Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR …

A key step of any CRISPR workflow is transfecting the gRNA and Cas9 into the target cells, but different transfection protocols have different advantages. This comprehensive guide covers all of the different …